High level expression and structural characterisation of herpes simplex virus type I transcriptional activator VP16 (alpha-trans inducing factor)
A M Spatz, S A Ali, M Auer, C Graf, M M Eibl, A Steinkasserer
Biochem Biophys Res Commun, 251(1), 235-8 (1998) .
1998
Full text: http://dx.doi.org/10.1006/bbrc.1998.9447
Abstract
We have developed a baculovirus expression system for the rapid and efficient production of large quantities (>10 mg/l) of VP16. The recombinant VP16 binds to a complex of host cell transcription factors and TAATGARAT motif. Secondary structure calculations from circular dichroism measurements indicate a content of 32.0 % alpha-helix and 17.5 % beta-sheet. This is the first structural CD analysis of VP16 which will be very useful for high-throughput assay development and mechanistic studies.
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